Molecular SNP markers are regions of DNA with variations. They help in selecting desired traits for plant breeding. Pm4b is a gene of interest that imparts the desired resistance against powdery mildew, a fungal disease in certain breeds of wheat. Samples with and without the Pm4b gene were taken for the identification of SNP markers … Continue reading “Identification of SNP Markers in Pm4b gene of Wheat – Part2”
Food – Primary source of essential nutrients to the whole world. When enquired on for one’s favorite food, we get to hear a wide variety of preferences from worldly cuisines. Most of these dish recipes would have wheat as a common ingredient. Wheat is a grass variety cultivated for its edible grains and is widely … Continue reading “Identification of SNP Markers in Pm4b gene of Wheat – Part1”
Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2): A 50-200 nm virion with a genome size of approximately 30000 nucleotides paused the normal and routine activities of the world by being the causative agent of Coronavirus Disease 2019 (COVID-19). Several questions came flooding when I started to know more about this novel coronavirus. The researcher in … Continue reading “Text Mining & NLP analysis of SARS-CoV-2”
Childhood memories of summer vacation involved watering the plants and taking care of the parent’s garden. It was filled with fun and intrigue as a new shoot with small leaves would sprout from a potato bud, or, out of nowhere a ”Touch me not” plant would grow, and when touched its leaves would shrink. Plants … Continue reading “Light switch of plants-HY5”
The picture above has a lot of beads and their colors are primarily shades of blue or pink. But clearly, there is one orange bead that stands out making a difference. In the same way, our DNA is a stitch and stretch of 4 primary chemical beads (bases)- Adenine (A), Thymine (T), Guanine (G), and … Continue reading “Demystifying the odd base conundrum”
I was watching this movie, where the protagonists were a race of human clones made from “stem cells” and they try to defend their lives from the native human race. You might have already heard of dolly the sheep, the first cloned animal out of an adult stem cell. Also, the infamous CRISPR babies whose … Continue reading “The silent biomarkers of stem cells- Small RNA”
Our planet accommodates a variety of life with their genes: from civilized humans to the colonized bacteria, from infamous mosquito to the cutest puppies, and from golden rice to the greenest algae. Studying these species helps us in understanding the genetics of the species and population diversity and also other biomedical and bio technical applications. … Continue reading “Strand NGS-Annotation toolbox for organisms”
Data quality is of utmost importance for accurate and valid downstream NGS analysis. Sequence artifacts contribute to erroneous interpretations in analysis. Focusing on this, we have introduced two quality checks to find Overrepresented Sequences and Adapter Content. Trim these sequence artifacts in further steps for accurate downstream analysis Strand NGS 3.4 now supports Agilent UMI … Continue reading “What’s new in Strand NGS 3.4”
A disease is considered to be ‘rare’ when it affects one in about 2000 individuals in the population. This, individually are although rare, collectively, the incidence could be very high causing a significant socio-economic burden. Arriving at a confirmatory diagnosis is a major challenge in these inherited disorders, which can significantly impact treatment and disease … Continue reading “Webinar on Diagnosis of Rare Diseases using NGS Based Multi-gene Testing- Case studies 18 Apr”
Background Next Generation Sequencing (NGS) technologies have remarkably revolutionized the medical and genomics research. The incremental cost reductions and size of the throughput at molecular resolution helped penetration and acceptance of the NGS methodologies into worldwide labs and clinics. The third generation wave of NGS technologies are knocking the doors to provide impetus to the … Continue reading “Ultra-sensitive variant calling and transcript quantification using Unique Molecular Identifiers”